Two-dimensional Phos-tag affinity electrophoresis
نویسندگان
چکیده
منابع مشابه
Quantitative protein profiling using two-dimensional gel electrophoresis, isotope-coded affinity tag labeling, and mass spectrometry.
Quantitative protein profiling is an essential part of proteomics and requires new technologies that accurately, reproducibly, and comprehensively identify and quantify the proteins contained in biological samples. We describe a new strategy for quantitative protein profiling that is based on the separation of proteins labeled with isotope-coded affinity tag reagents by two-dimensional gel elec...
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Protein phosphorylation is one of the most common post-translational modifications regulating many cellular processes. The phos-tag technology was combined with two-dimensional zymograms, which consisted of non-reducing IEF PAGE or NEPHGE in the first dimension and high resolution clear native electrophoresis (hrCNE) in the second dimension. The combination of these electrophoresis methods was ...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 2012
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.56.s21